In this work, by comprehensively analyzing the progresses in exosome separation strategies, we provide a panoramic view of current exosome isolation techniques, providing perspectives toward the development of novel approaches for high-efficient exosome isolation from various types of biological matrices. Over the past few decades, although a standardized exosome isolation method has still not become available, a number of techniques have been established through exploration of the biochemical and physicochemical features of exosomes.
Historically, the two main technical hindrances that have restricted the basic and applied researches of exosomes include, first, how to simplify the extraction and improve the yield of exosomes and, second, how to effectively distinguish exosomes from other extracellular vesicles, especially functional microvesicles. However, as in any natural science field, the in-depth investigation of exosomes relies heavily on technological advances.
Consequently, exosome-based disease diagnosis and therapeutic methods have been intensively investigated. In both physiological and pathological conditions, nearly all types of cells can release exosomes, which play important roles in cell communication and epigenetic regulation by transporting crucial protein and genetic materials such as miRNA, mRNA, and DNA. File import instruction AbstractĮxosomes are small extracellular vesicles with diameters of 30-150 nm. Select the file that you have just downloaded and select import option Reference Manager (RIS).
Available fromĬlick on Go to download the file. Progress, opportunity, and perspective on exosome isolation - efforts for efficient exosome-based theranostics. Yang D, Zhang W, Zhang H, Zhang F, Chen L, Ma L, Larcher LM, Chen S, Liu N, Zhao Q, Tran PHL, Chen C, Veedu RN, Wang T. ✉ Corresponding author: Tao Wang, wangtaomaryedu.cn Rakesh N Veedu, R. Changying Chen, changyingedu.cn. #These authors contribute equally to this work. Perron Institute for Neurological and Translational Science, Perth 6009, Australia The First Affiliated Hospital of Zheng Zhou University, Zhengzhou 450001, China.ġ1. School of Medicine, and Centre for Molecular and Medical Research, Deakin University, 3216, Australia.ġ0. School of Medicine, Wake Forest University, Winston Salem, NC 27101, USA.ĩ. General Practice Centre, Nanhai Hospital, Southern Medical University, 528244, Foshan, China.Ĩ.
Centre for Molecular Medicine and Innovative Therapeutics, Murdoch University, Perth 6150, Australia.ħ. School of Statistics, Henan University of Economics and Law, Zhengzhou 450046, China.Ħ. Guangdong Key Laboratory for Research and Development of Nature Drugs, School of Pharmacy, Guangdong Medical University, Zhanjiang 524023, China.ĥ. Henan Key Laboratory of Ion-beam Bioengineering, Zhengzhou University, Zhengzhou, China, 450000.Ĥ. School of Nursing, Zhengzhou University, Zhengzhou, 450001, China.ģ. Department of Neurosurgery of Hebi People's Hospital Hebi Neuroanatomical Laboratory, Hebi, 458030, China.Ģ. Tran 9, Changying Chen 10, Rakesh N Veedu 6,11, Tao Wang 2,6,11ġ.
Larcher 6, Suxiang Chen 6, Nan Liu 7, Qingxia Zhao 8, Phuong H.L. Review Progress, opportunity, and perspective on exosome isolation - efforts for efficient exosome-based theranosticsĭongbin Yang 1#, Weihong Zhang 2#, Huanyun Zhang 1, Fengqiu Zhang 3, Lanmei Chen 4, Lixia Ma 5, Leon M.